Locations of several novel 2-O-methylated nucleotides in human 28S rRNAReport as inadecuate

Locations of several novel 2-O-methylated nucleotides in human 28S rRNA - Download this document for free, or read online. Document in PDF available to download.

BMC Molecular Biology

, 3:1

First Online: 14 February 2002Received: 07 November 2001Accepted: 14 February 2002


BackgroundRibose 2-O-methylation, the most common nucleotide modification in mammalian rRNA, is directed by the C-D box small nucleolar RNAs snoRNAs. Thus far, more than fifty putative human rRNA methylation guide snoRNAs have been identified. For nine of these snoRNAs, the respective ribose methylations in human 28S rRNA have been only presumptive.

ResultsIn this study, the methylation state of human 28S rRNA in the positions predicted by the snoRNAs U21, U26, U31, U48, U50, U73, U74, U80 and U81 was assessed using reverse transcription-based methods and several novel 2-O-methylations were localized.

ConclusionsSeven novel ribose 2-O-methylated residues Am389, Am391, Gm1604, Gm1739, Gm2853, Cm3810, Gm4156, predicted by snoRNAs U26, U81, U80, U73, U50, U74 and U31, respectively have been localized in human 28S rRNA. The total number of 2-O-methylations in human rRNA is not yet known.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2199-3-1 contains supplementary material, which is available to authorized users.

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Author: Ana Rebane - Hanno Roomere - Andres Metspalu

Source: https://link.springer.com/

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