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Lipids in Health and Disease

, 1:1

First Online: 03 September 2002Received: 01 August 2002Accepted: 03 September 2002

Abstract

Anandamide N-arachidonoylethanolamine, AEA belongs to an emerging class of endogenous lipids, called -endocannabinoids-. A specific AEA membrane transporter AMT allows the import of this lipid and its degradation by the intracellular enzyme AEA hydrolase. Here, we show that synaptosomes from human, mouse and rat brain might be an ideal ex vivo system for the study of: i the accumulation of AEA in brain, and ii the pharmacological properties of AMT inhibitors. Using this ex vivo system, we demonstrate for the first time that glutamine and glutamate act as non-competitive inhibitors of AEA uptake by human, mouse and rat brain AMT.

Natalia Battista, Monica Bari contributed equally to this work.

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Autor: Natalia Battista - Monica Bari - Alessandro Finazzi-Agrò - Mauro Maccarrone

Fuente: https://link.springer.com/







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