Combined Analysis of Endothelial, Hematopoietic, and Mesenchymal Stem Cell Compartments Shows Simultaneous but Independent Effects of Age and Heart DiseaseReportar como inadecuado




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Stem Cells International - Volume 2017 2017, Article ID 5237634, 13 pages - https:-doi.org-10.1155-2017-5237634

Research Article

Institute of Cardiology of Rio Grande do Sul, Fundação Universitária de Cardiologia, Av. Princesa Isabel 395, 90040-371 Porto Alegre, RS, Brazil

Universidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite 245, 90050-170 Porto Alegre, RS, Brazil

Laboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, Av. Farroupilha 8001, 92425-900 Canoas, RS, Brazil

Correspondence should be addressed to Nance Beyer Nardi

Received 5 March 2017; Revised 24 May 2017; Accepted 6 June 2017; Published 27 July 2017

Academic Editor: Tao-Sheng Li

Copyright © 2017 Carine Ghem et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Clinical trials using stem cell therapy for heart diseases have not reproduced the initial positive results obtained with animal models. This might be explained by a decreased regenerative capacity of stem cells collected from the patients. This work aimed at the simultaneous investigation of endothelial stem-progenitor cells EPCs, mesenchymal stem-progenitor cells MSCs, and hematopoietic stem-progenitor cells HSCs in sternal bone marrow samples of patients with ischemic or valvular heart disease, using flow cytometry and colony assays. The study included 36 patients referred for coronary artery bypass grafting or valve replacement surgery. A decreased frequency of stem cells was observed in both groups of patients. Left ventricular dysfunction, diabetes, and intermediate risk in EuroSCORE and SYNTAX score were associated with lower EPCs frequency, and the use of aspirin and β-blockers correlated with a higher frequency of HSCs and EPCs, respectively. Most importantly, the distribution of frequencies in the three stem cell compartments showed independent patterns. The combined investigation of the three stem cell compartments in patients with cardiovascular diseases showed that they are independently affected by the disease, suggesting the investigation of prognostic factors that may be used to determine when autologous stem cells may be used in cell therapy.





Autor: Carine Ghem, Lucinara Dadda Dias, Roberto Tofani Sant’Anna, Renato A. K. Kalil, Melissa Markoski, and Nance Beyer Nardi

Fuente: https://www.hindawi.com/



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