Lipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acidsReportar como inadecuado




Lipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acids - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 CSGA - Centre des Sciences du Goût et de l-Alimentation Dijon 2 EA 2521, JE2521, IFR143 - Biologie, Ingénierie et Imagerie de la Greffe de Cornée 3 CHU Dijon

Abstract : BACKGROUND: The assessment of blood lipids is very frequent in clinical research as it is assumed to reflect the lipid composition of peripheral tissues.
Even well accepted such relationships have never been clearly established.
This is particularly true in ophthalmology where the use of blood lipids has become very common following recent data linking lipid intake to ocular health and disease.
In the present study, we wanted to determine in humans whether a lipidomic approach based on red blood cells could reveal associations between circulating and tissue lipid profiles.
To check if the analytical sensitivity may be of importance in such analyses, we have used a double approach for lipidomics.
METHODOLOGY AND PRINCIPAL FINDINGS: Red blood cells, retinas and optic nerves were collected from 9 human donors.
The lipidomic analyses on tissues consisted in gas chromatography and liquid chromatography coupled to an electrospray ionization source-mass spectrometer LC-ESI-MS.
Gas chromatography did not reveal any relevant association between circulating and ocular fatty acids except for arachidonic acid whose circulating amounts were positively associated with its levels in the retina and in the optic nerve.
In contrast, several significant associations emerged from LC-ESI-MS analyses.
Particularly, lipid entities in red blood cells were positively or negatively associated with representative pools of retinal docosahexaenoic acid DHA, retinal very-long chain polyunsaturated fatty acids VLC-PUFA or optic nerve plasmalogens.
CONCLUSIONS AND SIGNIFICANCE: LC-ESI-MS is more appropriate than gas chromatography for lipidomics on red blood cells, and further extrapolation to ocular lipids.
The several individual lipid species we have identified are good candidates to represent circulating biomarkers of ocular lipids.
However, further investigation is needed before considering them as indexes of disease risk and before using them in clinical studies on optic nerve neuropathies or retinal diseases displaying photoreceptors degeneration.






Autor: Acar Niyazi - Olivier Berdeaux - Stéphane Grégoire - Stéphanie Cabaret - Lucy Martine - Philippe Gain - Gilles Thuret - Cather

Fuente: https://hal.archives-ouvertes.fr/



DESCARGAR PDF




Documentos relacionados