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1 MSC - Matière et Systèmes Complexes 2 LVTS - Laboratoire de Recherche Vasculaire Translationnelle 3 LIOAD - Laboratoire d-ingénierie osteo-articulaire et dentaire

Abstract : Tissue engineering strategies, such as cellularized scaffolds approaches, have been explored for cartilage replacement. The challenge, however, remains to produce a cartilaginous tissue incorporating functional chondrocytes and being large and thick enough to be compatible with the replacement of articular defects. Here, we achieved unprecedented cartilage tissue production into a porous polysaccharide scaffold by combining of efficient magnetic condensation of mesenchymal stem cells, and dynamic maturation in a bioreactor. In optimal conditions, all the hallmarks of chondrogenesis were enhanced with a 50-fold increase in collagen II expression compared to negative control, an overexpression of aggrecan and collagen XI, and a very low expression of collagen I and RUNX2. Histological staining showed a large number of cellular aggregates, as well as an increased proteoglycan synthesis by chondrocytes. Interestingly, electron microscopy showed larger chondrocytes and a more abundant extracellular matrix. In addition, the periodicity of the neosynthesized collagen 2 fibers matched that of collagen II. These results represent a major step forward in replacement tissue for cartilage defects.

Keywords : Magnetic mesenchymal stem cells Chondrogenesis Cartilage defect Bioreactor Tissue engineering

Autor: Nathalie Luciani - Vicard Du - Florence Gazeau - Alain Richert - Didier Letourneur - Catherine Le Visage - Claire Wilhelm -



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