Long-term immune reconstitution and T cell repertoire analysis after autologous hematopoietic stem cell transplantation in systemic sclerosis patientsReportar como inadecuado




Long-term immune reconstitution and T cell repertoire analysis after autologous hematopoietic stem cell transplantation in systemic sclerosis patients - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1 Service d-Immunopathologie Hôpital Saint-Louis, Paris 2 A2T - Alloimmunité-Autoimmunité-Transplantation 3 Faculdade de Medicina de Ribeirão Preto 4 UPMC - Université Pierre et Marie Curie - Paris 6 5 Laboratoire d-Hématologie et d-Immunologie CHU Saint-Antoine 6 Hôpital Pierre Zobda-Quitman Fort-de-France, Martinique 7 Eurocord 8 CSM - Centre Scientifique de Monaco

Abstract : The determinants of clinical responses after autologous hematopoietic stem cell transplantation aHSCT in systemic sclerosis SSc are still unraveled. We analyzed long-term immune reconstitution IR and T cell receptor TCR repertoire diversity in 10 SSc patients, with at least 6 years simultaneous clinical and immunological follow-up after aHSCT. Patients were retrospectively classified as long-term responders A, n = 5 or non-responders B, n = 5, using modified Rodnan-s skin score mRSS and forced vital capacity FVC%. All patients had similar severe SSc before aHSCT. Number of reinjected CD34 + cells was higher in group B versus A P = 0.02. Long-term mRSS fall >25% was more pronounced in group A P = 0.004, the only to improve long-term FVC% >10% P = 0.026. There was an overall trend toward increased of T cell reconstitution in group B versus A. B cells had a positive linear regression slope in group A LRS = 11.1 and negative in group B LRS = −11.6. TCR repertoire was disturbed before aHSCT and the percentage of polyclonal families significantly increased at long-term P = 0.046, with no difference between groups. Despite improved skin score after aHSCT in all SSc patients, pretransplant B cell clonal expansion and faster post-transplant T cell IR in long-term non-responder-relapsing patients call for new therapeutic protocols guided by IR analysis to improve their outcome.

Keywords : Hematopoietic stem cell transplantation Systemic sclerosis T cell repertoire Immune reconstitution





Autor: Dominique Farge - Lucas C. M. Arruda - Fanny Brigant - Emmanuel Clave - Corinne Douay - Zora Marjanovic - Christophe Deligny - Gu

Fuente: https://hal.archives-ouvertes.fr/



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