First report of L1014F-kdr mutation in Culex pipiens complex from Morocco.Reportar como inadecuado

First report of L1014F-kdr mutation in Culex pipiens complex from Morocco. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 Institut Pasteur du Maroc 2 Laboratoire de Virologie Microbiologie & Qualité- Eco-toxicologie & Biodiversité Casablanca 3 Laboratoire d-Entomologie médicale Rabat 4 Arbovirus et Insectes Vecteurs

Abstract : BackgroundMosquitoes of the Culex pipiens complex, competent vectors for West Nile virus WNV and Rift Valley fever virus RVFV are widely targeted by insecticide treatments. The intensive application of chemical insecticides led to the development of resistance in many insects including Culex pipiens mosquitoes. The absence of data on resistance mechanisms in Morocco allow us to assess the levels of lambda-cyhalothrin resistance and the frequency of the mutated gene L1014F kdr in different forms of Cx. pipiens complex from three regions of Morocco.MethodsMosquito adults were reared from immature stages collected in three different regions in Morocco Tangier, Casablanca and Marrakech. Standard WHO insecticide susceptibility tests were conducted on adults emerged from collected larvae. Specimens were identified as belonging to the Culex pipiens complex using a multiplex PCR assay with diagnostic primers designed from the flanking region of microsatellite CQ11. Identified mosquitoes were then tested for the presence of the L1014F kdr mutation using PCR assay.ResultsOur results showed that 21% of the tested population has a resistance to lambda-cyhalothrin. The molecular identification of survivors shows that 43% belonged to the Cx. pipiens pipiens and only 9.5% to the Cx. pipiens molestus form. On the other hand, 416 specimens were screened for the L1014F kdr mutation. L1014F mutation was detected in different forms of Cx. pipiens in different sites. The frequency of L1014F mutation was similar between the Cx. pipiens pipiens form and hybrid form, while it was lower in the Cx. pipiens molestus form. The presence of the L1014F kdr allele was significantly associated with resistance to lambda-cyhalothrin in Cx. pipiens pipiens P 

Autor: Meriem Bkhache - Fatim-Zohra Tmimi - Omar Charafeddine - Chafika Faraj - Anna-Bella Failloux - M-Hammed Sarih -



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