The Immunomodulatory Effects of Nidus Vespae on Human Peripheral Blood Immune Cells In VitroReportar como inadecuado




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Evidence-Based Complementary and Alternative Medicine - Volume 2015 2015, Article ID 705308, 6 pages -

Research ArticleClinical Oncology Laboratory, Changzhou Tumor Hospital Affiliated to Soochow University, Huaide Road 44, Changzhou 213002, China

Received 18 March 2015; Revised 9 July 2015; Accepted 21 July 2015

Academic Editor: Michał Tomczyk

Copyright © 2015 Ming Zhu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nidus Vespae has been used in traditional Chinese medicine TCM to treat various cancers, but the underlying mechanisms were not yet clarified. This study was to investigate the effect of Nidus Vespae decoction NVD on tumor cell viability and immunoregulating functions of human peripheral blood immune cells. The effects on tumor cell viability, peripheral blood mononuclear cell PBMC proliferation activity, and the tumor cell phagocytosis of monocytes were evaluated by cell counting kit-8. Tumor-killing activity of cytotoxic T lymphocyte CTL was analyzed by

Cr releasing assay. IgG production of B cells and cytokine TNF-α and IL-6 secretion of monocytes were determined by ELISA method. Data showed that NVD has no significant inhibiting effects on gastric cancer cells growth. Nevertheless, it could obviously promote PBMC proliferation in a time- and concentration-dependent manner. After treatment with NVD, the CTL cytotoxicity against SGC-7901 was significantly greater than control. The TNF-α and IL-6 secretion of monocytes and the IgG production of B cells also increased remarkably. Furthermore, NVD could significantly promote the phagocytosis of monocytes on tumor cells. These results suggest that NVD appears to have an immunoenhancing effect on immune cells, indicating that Nidus Vespae is worth exploring for immunomodulatory effects in tumor treatment.





Autor: Ming Zhu, Yang Ling, Qiufeng Qi, Yaping Zhang, Yanqing Bao, and Yongping Liu

Fuente: https://www.hindawi.com/



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