PKA Inhibitor H89 N-2-p-bromocinnamylamino-ethyl-5-isoquinolinesulfonamide Attenuates Synaptic Dysfunction and Neuronal Cell Death following Ischemic InjuryReportar como inadecuado




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Neural Plasticity - Volume 2015 2015, Article ID 374520, 13 pages -

Research Article

Department of Anatomy, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea

BK21 Plus Project for Medical Sciences and Brain Research Institute, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea

Received 8 January 2015; Revised 27 February 2015; Accepted 17 March 2015

Academic Editor: Young W. Yoon

Copyright © 2015 Juhyun Song et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The cyclic AMP-dependent protein kinase PKA, which activates prosurvival signaling proteins, has been implicated in the expression of long-term potentiation and hippocampal long-term memory. It has come to light that H89 commonly known as the PKA inhibitor have diverse roles in the nervous system that are unrelated to its role as a PKA inhibitor. We have investigated the role of H89 in ischemic and reperfusion injury. First, we examined the expression of postsynaptic density protein 95 PSD95, microtubule-associated protein 2 MAP2, and synaptophysin in mouse brain after middle cerebral artery occlusion injury. Next, we examined the role of H89 pretreatment on the expression of brain-derived neurotrophic factor BDNF, PSD95, MAP2, and the apoptosis regulators Bcl2 and cleaved caspase-3 in cultured neuroblastoma cells exposed to hypoxia and reperfusion injury. In addition, we investigated the alteration of AKT activation in H89 pretreated neuroblastoma cells under hypoxia and reperfusion injury. The data suggest that H89 may contribute to brain recovery after ischemic stroke by regulating neuronal death and proteins related to synaptic plasticity.





Autor: Juhyun Song, So Yeong Cheon, Won Taek Lee, Kyung Ah Park, and Jong Eun Lee

Fuente: https://www.hindawi.com/



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