Biosurfactant-Producing Lactobacilli: Screening, Production Profiles, and Effect of Medium CompositionReportar como inadecuado

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Applied and Environmental Soil ScienceVolume 2011 2011, Article ID 201254, 9 pages

Research ArticleInstitute for Biotechnology and Bioengineering IBB, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal

Received 22 June 2011; Accepted 28 September 2011

Academic Editor: Elena Tamburini

Copyright © 2011 Eduardo J. Gudiña et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Biosurfactant production was screened in four lactobacilli strains. The highest biosurfactant production excreted and cell-bound biosurfactants was achieved with Lactobacillus paracasei ssp. paracasei A20, a strain isolated from a Portuguese dairy plant, with a decrease in the surface tension of 6.4 mN m

and 22.0 mN m

, respectively. Biosurfactant production by this strain was evaluated under different culture broth compositions. The use of different nitrogen sources revealed that yeast extract is essential for bacterial growth, while peptone is crucial for biosurfactant synthesis. For biosurfactant production, the use of peptone and meat extract yielded a higher production when compared to the standard medium, with a surface tension reduction of 24.5 mN m

. Furthermore, experiments were also conducted in a reactor with pH and temperature control. Biomass and biosurfactant production in bioreactor was higher comparing with the experiments conducted in shake flaks. The optimization procedure adopted in the current work was found to improve the biosurfactant production and opened new perspectives for the use of L. paracasei ssp. paracasei A20 as a promising biosurfactant-producer.

Autor: Eduardo J. Gudiña, José A. Teixeira, and Lígia R. Rodrigues



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