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* Corresponding author 1 Angiogénèse embryonnaire et pathologique 2 Beijing Union Medical College 3 School of Life Sciences 4 Genentech 5 Mécanismes moléculaires de l-angiogénèse

Abstract : Netrin-1 is a bifunctional axonal guidance cue, capable of attracting or repelling developing axons via activation of receptors of the deleted in colorectal cancer DCC and uncoordinated 5 UNC5 families, respectively. In addition to its role in axon guidance, Netrin-1 has been implicated in angiogenesis, where it may also act as a bifunctional cue. Attractive effects of Netrin-1 on endothelial cells appear to be mediated by an as yet unknown receptor, while repulsion of developing blood vessels in mouse embryos is mediated by the UNC5B receptor. To explore evolutionary conservation of vascular UNC5B expression and function, we have cloned the chick unc5b homologue. Chick and quail embryos showed unc5b expression in arterial EC and sprouting angiogenic capillaries. To test if Netrin-1 displayed pro- or anti-angiogenic activities in the avian embryo, we grafted cell lines expressing recombinant chick or human Netrin-1 at different stages of development. Netrin-1 expressing cells inhibited angiogenic sprouting of unc5b expressing blood vessels, but had no pro-angiogenic activity at any stage of development examined. Netrin-1 also had no effect on the recruitment of circulating endothelial precursor cells. Taken together, these data indicate that vascular unc5b expression and function is conserved between chick and mice.

Keywords : vessel guidance UNC5B angiogenesis sprouting CEC-EPC





Autor: Karine Bouvrée - Bruno Larrivée - Xiang Lv - Li Yuang - Benjamin Delafarge - Catarina Freitas - Thomas Mathivet - Christiane Br

Fuente: https://hal.archives-ouvertes.fr/



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