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Biotechnology Research InternationalVolume 2011 2011, Article ID 737636, 8 pages

Research ArticleVision MGM Agri-Tech and Research Institute Pvt. Ltd., C-122 HIG, Baramunda, Bhubaneswar-751 003, Orissa, India

Received 20 May 2010; Accepted 16 July 2010

Academic Editor: Masaru Ohme-Takagi

Copyright © 2011 Premananda Das. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Somatic embryogenesis was achieved in four leguminous tree species, that is, Acacia catechu, Acacia arabica, Hardwickia binata, and Dalbergia sissoo using immature zygotic embryos as explants on Murashige and Skoog MS medium supplemented with 0.25–1.0 mg-l Kn kinetin and 2.0–3.0 mg-l 2,4-D 2,4-dichlorophenoxyacetic acid or NAA 1-napthaleneacetic acid and 3% sucrose. MS medium containing 2.0 mg-l 2,4-D and 1.0–1.5 mg-l Kn was noted to be most effective in inducing friable embryogenic callus FEC; the number of somatic embryos per culture varied in MS medium supplemented with 1.0–2.0 mg-l 2,4-D or NAA and 0.25–1.5 mg-l kinetin. The maximum number of somatic embryos was obtained in MS medium containing 1.5–2.0 mg-l 2,4-D or NAA and 1.0–1.5 mg-l kinetin; proliferation of embryogenic calli was enhanced in cultures having 1.0–2.0 mg-l 2,4-D, 1.0–1.5 mg-l kinetin, and 400–600 mg-l L-Proline. The somatic embryos in various shapes and sizes after the first subculture on MS medium supplemented with 0.1 mg-l IAA and 0.25 mg-l BA; developed shoots and rooted in strength MS medium supplemented with 0.1 mg-l IBA or IAA. The somatic embryo-derived plantlets were transferred to the field after being hardened in the climate-controlled hardening chamber.

Autor: Premananda Das



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