Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algaeReportar como inadecuado




Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 Department of Plant Sciences 2 LBI2M - Laboratoire de Biologie Intégrative des Modèles Marins

Abstract : Laser capture microdissection LCM facilitates the isolation of individual cells from tissue sections, and when combined with RNA amplification techniques, it is an extremely powerful tool for examining genome-wide expression profiles in specific cell-types. LCM has been widely used to address various biological questions in both animal and plant systems, however, no attempt has been made so far to transfer LCM technology to macroalgae. Macroalgae are a collection of widespread eukaryotes living in fresh and marine water. In line with the collective effort to promote molecular investigations of macroalgal biology, here we demonstrate the feasibility of using LCM and cell-specific transcriptomics to study development of the brown alga Ectocarpus siliculosus. We describe a workflow comprising cultivation and fixation of algae on glass slides, laser microdissection, and RNA amplification. To illustrate the effectiveness of the procedure, we show qPCR data and metrics obtained from cell-specific transcriptomes generated from both upright and prostrate filaments of Ectocarpus.

Keywords : laser capture microdissection cell-specific transcriptomics cell differentiation seaweed brown algae





Autor: Denis Saint-Marcoux - Bernard Billoud - Jane A. Langdale - Bénédicte Charrier -

Fuente: https://hal.archives-ouvertes.fr/



DESCARGAR PDF




Documentos relacionados