Quantification of Small Non-Coding RNAs Allows an Accurate Comparison of miRNA Expression ProfilesReportar como inadecuado




Quantification of Small Non-Coding RNAs Allows an Accurate Comparison of miRNA Expression Profiles - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of Biomedicine and BiotechnologyVolume 2009 2009, Article ID 659028, 9 pages

Methodology Report

Gene Expression and Microarrays Laboratory, Bambino Gesú Children's Hospital, P. za S. Onofrio 4, 00165 Rome, Italy

IRCCS-CSS, San Giovanni Rotondo and CSS-Mendel Institute, Viale Regina Margherita 261, 00198 Rome, Italy

Department of Experimental Medicine and Pathology, University La Sapienza, Viale Regina Elena 324, 00161 Rome, Italy

Scientific Directorate, Bambino Gesú Children's Hospital, P. za S. Onofrio 4, 00165 Rome, Italy

Received 1 January 2009; Revised 17 April 2009; Accepted 4 June 2009

Academic Editor: Daniel Gautheret

Copyright © 2009 Andrea Masotti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

MicroRNAs miRNAs are highly conserved 22-mer RNA molecules, encoded by plants and animals that regulate the expression of genes binding to the -UTR of specific target mRNAs. The amount of miRNAs in a total RNA sample depends on the recovery efficiency that may be significantly affected by the different purification methods employed. Traditional approaches may be inefficient at recovering small RNAs, and common spectrophotometric determination is not adequate to quantify selectively these low molecular weight LMW species from total RNA samples. Here, we describe the use of qualitative and quantitative lab-on-a-chip tools for the analysis of these LMW RNA species. Our data emphasize the close correlation of LMW RNAs with the expression levels of some miRNAs. We therefore applied our result to the comparison of some miRNA expression profiles in different tissues. Finally, the methods we used in this paper allowed us to analyze the efficiency of extraction protocols, to study the small but significant differences among various preparations and to allow a proper comparison of some miRNA expression profiles in various tissues.





Autor: Andrea Masotti, Viviana Caputo, Letizia Da Sacco, Antonio Pizzuti, Bruno Dallapiccola, and Gian Franco Bottazzo

Fuente: https://www.hindawi.com/



DESCARGAR PDF




Documentos relacionados