Planktonic foraminifera-derived environmental DNA extracted from abyssal sediments preserves patterns of plankton macroecologyReport as inadecuate




Planktonic foraminifera-derived environmental DNA extracted from abyssal sediments preserves patterns of plankton macroecology - Download this document for free, or read online. Document in PDF available to download.

1 MARUM, Center for Marine Environmental Sciences 2 University of Geneva Switzerland 3 University of Edinburg Edinburg 4 University of St Andrews Fife, Scotland, UK 5 Okinawa Institute of Science and Technology 6 KU - University of Copenhagen 7 EPEP - Evolution des Protistes et Ecosystèmes Pélagiques ADMM - Adaptation et diversité en milieu marin

Abstract : Deep-sea sediments constitute a unique archive of ocean change, fueled by a permanent rain of mineral and organic remains from the surface ocean. Until now, paleo-ecological analyses of this archive have been mostly based on information from taxa leaving fossils. In theory, environmental DNA eDNA in the sediment has the potential to provide information on non-fossilized taxa, allowing more comprehensive interpretations of the fossil record. Yet, the process controlling the transport and deposition of eDNA onto the sediment and the extent to which it preserves the features of past oceanic biota remains unknown. Planktonic foraminifera are the ideal taxa to allow an assessment of the eDNA signal modification during deposi-tion because their fossils are well preserved in the sediment and their morphological taxonomy is documented by DNA barcodes. Specifically, we re-analyze foraminiferal-specific metabarcodes from 31 deep-sea sediment samples, which were shown to contain a small fraction of sequences from planktonic foraminifera. We confirm that the largest portion of the metabarcode originates from benthic bottom-dwelling foraminifera, representing the in situ community, but a small portion < 10 % of the metabarcodes can be unambiguously assigned to planktonic taxa. These organisms live exclusively in the surface ocean and the recovered barcodes thus represent an allochthonous component deposited with the rain of organic remains from the surface ocean. We take advantage of the planktonic foraminifera portion of the metabar-codes to establish to what extent the structure of the surface ocean biota is preserved in sedimentary eDNA. We show that planktonic foraminifera DNA is preserved in a range of marine sediment types, the composition of the recovered eDNA metabarcode is replicable and that both the similarity structure and the diversity pattern are preserved. Our results suggest that sedimentary eDNA could preserve the ecological structure of the entire pelagic community, including non-fossilized taxa, thus opening new avenues for paleoceano-graphic and paleoecological studies.





Author: Raphaël Morard - Franck Lejzerowicz - Kate F. Darling - Béatrice Lecroq-Bennet - Mikkel Winther Pedersen - Ludovic Orlando - Ja

Source: https://hal.archives-ouvertes.fr/



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