MUBII-TB-DB: a database of mutations associated with antibiotic resistance in Mycobacterium tuberculosis.Report as inadecuate

MUBII-TB-DB: a database of mutations associated with antibiotic resistance in Mycobacterium tuberculosis. - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 LBBE - Laboratoire de Biométrie et Biologie Evolutive 2 CIRI - Centre International de Recherche en Infectiologie 3 Hospices Civils de Lyon

Abstract : BACKGROUND: Tuberculosis is an infectious bacterial disease caused by Mycobacterium tuberculosis. It remains a major health threat, killing over one million people every year worldwide. An early antibiotic therapy is the basis of the treatment, and the emergence and spread of multidrug and extensively drug-resistant mutant strains raise significant challenges. As these bacteria grow very slowly, drug resistance mutations are currently detected using molecular biology techniques. Resistance mutations are identified by sequencing the resistance-linked genes followed by a comparison with the literature data. The only online database is the TB Drug Resistance Mutation database TBDReaM database; however, it requires mutation detection before use, and its interrogation is complex due to its loose syntax and grammar.Description: The MUBII-TB-DB database is a simple, highly structured text-based database that contains a set of Mycobacterium tuberculosis mutations DNA and proteins occurring at seven loci: rpoB, pncA, katG; mabAfabG1-inhA, gyrA, gyrB, and rrs. Resistance mutation data were extracted after the systematic review of MEDLINE referenced publications before March 2013. MUBII analyzes the query sequence obtained by PCR-sequencing using two parallel strategies: i a BLAST search against a set of previously reconstructed mutated sequences and ii the alignment of the query sequences DNA and its protein translation with the wild-type sequences. The post-treatment includes the extraction of the aligned sequences together with their descriptors position and nature of mutations. The whole procedure is performed using the internet. The results are graphs alignments and text description of the mutation, therapeutic significance. The system is quick and easy to use, even for technicians without bioinformatics training. CONCLUSION: MUBII-TB-DB is a structured database of the mutations occurring at seven loci of major therapeutic value in tuberculosis management. Moreover, the system provides interpretation of the mutations in biological and therapeutic terms and can evolve by the addition of newly described mutations. Its goal is to provide easy and comprehensive access through a client-server model over the Web to an up-to-date database of mutations that lead to the resistance of M. tuberculosis to antibiotics.

Keywords : Tuberculosis Antibiotics Mutation database Sequence database Web

Author: Jean-Pierre Flandrois - Gérard Lina - Oana Dumitrescu -



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