The Ehrlich Tumor Induces Pain-Like Behavior in Mice: A Novel Model of Cancer Pain for Pathophysiological Studies and Pharmacological ScreeningReport as inadecuate




The Ehrlich Tumor Induces Pain-Like Behavior in Mice: A Novel Model of Cancer Pain for Pathophysiological Studies and Pharmacological Screening - Download this document for free, or read online. Document in PDF available to download.

BioMed Research InternationalVolume 2013 2013, Article ID 624815, 12 pages

Research Article

Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Rod. Celso Garcia Cid KM480 PR445, 86051-990 Londrina, PR, Brazil

Departamento de Ciências Fisiológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Rod. Celso Garcia Cid KM480 PR445, 86051-990 Londrina, PR, Brazil

Departamento de Enfermagem, Centro de Ciências da Saúde, Universidade Estadual de Londrina, Avenida Robert Koch 60, 86038-350 Londrina, PR, Brazil

Departamento Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Estadual de Londrina, Avenida Robert Koch 60, 86038-350 Londrina, PR, Brazil

Received 29 April 2013; Accepted 10 July 2013

Academic Editor: Monica Fedele

Copyright © 2013 Cassia Calixto-Campos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The Ehrlich tumor is a mammary adenocarcinoma of mice that can be developed in solid and ascitic forms depending on its administration in tissues or cavities, respectively. The present study investigates whether the subcutaneous plantar administration of the Ehrlich tumor cells induces pain-like behavior and initial pharmacological susceptibility characteristics. The Ehrlich tumor cells 1 × 10

–10

cells induced dose-dependent mechanical hyperalgesia electronic version of the von Frey filaments, paw edema-tumor growth caliper, and flinches compared with the saline group between days 2 and 12. There was no difference between doses of cells regarding thermal hyperalgesia in the hot-plate test. Indomethacin a cyclooxygenase inhibitor and amitriptyline hydrochloride a tricyclic antidepressant treatments did not affect flinches or thermal and mechanical hyperalgesia. On the other hand, morphine an opioid inhibited the flinch behavior and the thermal and mechanical hyperalgesia. These effects of morphine on pain-like behavior were prevented by naloxone an opioid receptor antagonist treatment. None of the treatments affected paw edema-tumor growth. The results showed that, in addition to tumor growth, administration of the Ehrlich tumor cells may represent a novel model for the study of cancer pain, specially the pain that is susceptible to treatment with opioids, but not to cyclooxygenase inhibitor or to tricyclic antidepressant.





Author: Cassia Calixto-Campos, Ana C. Zarpelon, Mab Corrêa, Renato D. R. Cardoso, Felipe A. Pinho-Ribeiro, Rubens Cecchini, Estefan

Source: https://www.hindawi.com/



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