Identification of Putative Ortholog Gene Blocks Involved in Gestant and Lactating Mammary Gland Development: A Rodent Cross-Species Microarray Transcriptomics ApproachReport as inadecuate

Identification of Putative Ortholog Gene Blocks Involved in Gestant and Lactating Mammary Gland Development: A Rodent Cross-Species Microarray Transcriptomics Approach - Download this document for free, or read online. Document in PDF available to download.

International Journal of GenomicsVolume 2013 2013, Article ID 624681, 12 pages

Research Article

Unit of Medical Research in Nutrition, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Avenida Cuauhtémoc 330, Col. Doctores, Delegación Cuauhtémoc, 06725 Mexico City, Mexico

Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s-n, Col. Casco de Santo Tomas, Delegación Miguel Hidalgo, 11340 Mexico City, Mexico

Subdirección de Enseñanza e Investigación, Centro Médico Nacional 20 de Noviembre, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, San Lorenzo 502 2 piso, Col. Del Valle, Delegación Benito Juárez, 03100 Mexico City, Mexico

Genomic Sciences Center, Universidad Nacional Autónoma de México, Avenida Universidad s-n, Col. Chamilpa, 62210 Cuernavaca, Morelos, Mexico

Department of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1 Col. Sección XVI, 140080 Tlalpan, Mexico City, DF, Mexico

Microarray Unit, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Circuito Exterior s-n, Ciudad Universitaria, Delegación Coyoacán, 04510 Mexico City, Mexico

Received 21 May 2013; Revised 3 September 2013; Accepted 4 September 2013

Academic Editor: Elena Pasyukova

Copyright © 2013 Maricela Rodríguez-Cruz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The mammary gland MG undergoes functional and metabolic changes during the transition from pregnancy to lactation, possibly by regulation of conserved genes. The objective was to elucidate orthologous genes, chromosome clusters and putative conserved transcriptional modules during MG development. We analyzed expression of 22,000 transcripts using murine microarrays and RNA samples of MG from virgin, pregnant, and lactating rats by cross-species hybridization. We identified 521 transcripts differentially expressed; upregulated in early 78% and midpregnancy 89% and early lactation 64%, but downregulated in mid-lactation 61%. Putative orthologous genes were identified. We mapped the altered genes to orthologous chromosomal locations in human and mouse. Eighteen sets of conserved genes associated with key cellular functions were revealed and conserved transcription factor binding site search entailed possible coregulation among all eight block sets of genes. This study demonstrates that the use of heterologous array hybridization for screening of orthologous gene expression from rat revealed sets of conserved genes arranged in chromosomal order implicated in signaling pathways and functional ontology. Results demonstrate the utilization power of comparative genomics and prove the feasibility of using rodent microarrays to identification of putative coexpressed orthologous genes involved in the control of human mammary gland development.

Author: Maricela Rodríguez-Cruz, Ramón M. Coral-Vázquez, Gabriel Hernández-Stengele, Raúl Sánchez, Emmanuel Salazar, Fausto San



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