Derivative Spectrophotometric Methods for the Analysis and Stability Studies of Colistin SulphateReport as inadecuate

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Journal of Chemistry - Volume 2015 2015, Article ID 624316, 5 pages -

Research ArticleDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, P.O. Box 1996, Khartoum, Sudan

Received 1 September 2015; Revised 29 November 2015; Accepted 29 November 2015

Academic Editor: Jean-Luc Blin

Copyright © 2015 Mohammed Mutasim Elimam et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Simple spectrophotometric methods were developed for the quantitative determination of colistin sulphate in bulk and dosage forms. The methods were based on the measurement of first and second derivative spectra of colistin sulphate at 298 nm and 318 nm, respectively. Beer’s law was obeyed in the concentration range 800–4000 IU-mL with good correlation coefficient not less than 0.998 for both methods. The developed first derivative spectrophotometric method was then selected to study the degradation behavior of colistin sulphate in alkaline media at different temperatures as the second derivative method failed to give reproducible results for the stability study. The pH-rate profile indicates a first-order dependence of the degradation rate on OH

at pH ranging between 8 and 11. The obtained results for the photochemical study reflected photostability of colistin sulphate.

Author: Mohammed Mutasim Elimam, Shaza Wagiealla Shantier, Elrasheed Ahmed Gadkariem, and Magdi Awadalla Mohamed



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