Andrographolide Inhibits Proliferation and Metastasis of SGC7901 Gastric Cancer CellsReport as inadecuate




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BioMed Research International - Volume 2017 2017, Article ID 6242103, 10 pages - https:-doi.org-10.1155-2017-6242103

Research Article

Department of Gastroenterology, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, China

Department of Gastroenterology, The Second Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou, Zhejiang, China

Tumor Institute of Integrative Medicine, Zhejiang Provincial Academy of Traditional Chinese Medicine, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, China

Correspondence should be addressed to Wensheng Pan

Received 9 September 2016; Revised 12 November 2016; Accepted 13 December 2016; Published 18 January 2017

Academic Editor: Hushan Yang

Copyright © 2017 Lei Dai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To explore the mechanisms by which andrographolide inhibits gastric cancer cell proliferation and metastasis, we employed the gastric cell line SGC7901 to investigate the anticancer effects of andrographolide. The cell survival ratio, cell migration and invasion, cell cycle, apoptosis, and matrix metalloproteinase activity were assessed. Moreover, western blotting and real-time PCR were used to examine the protein expression levels and the mRNA expression levels, respectively. The survival ratio of cells decreased with an increasing concentration of andrographolide in a dose-dependent manner. Consistent results were also obtained using an apoptosis assay, as detected by flow cytometry. The cell cycle was blocked at the G2-M2 phase by andrographolide treatment, and the proportion of cells arrested at G1-M was enhanced as the dose increased. Similarly, wound healing and Transwell assays showed reduced migration and invasion of the gastric cancer cells at various concentrations of andrographolide. Andrographolide can inhibit cell proliferation, invasion, and migration, block the cell cycle, and promote apoptosis in SGC7901 cells. The mechanisms may include upregulated expression of Timp-1-2, cyclin B1, p-Cdc2, Bax, and Bik and downregulated expression of MMP-2-9 and antiapoptosis protein Bcl-2.





Author: Lei Dai, Gang Wang, and Wensheng Pan

Source: https://www.hindawi.com/



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