Back-translation for discovering distant protein homologies in the presence of frameshift mutationsReport as inadecuate




Back-translation for discovering distant protein homologies in the presence of frameshift mutations - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 SEQUOIA2 - Algorithms for large scale sequence analysis LIFL - Laboratoire d-Informatique Fondamentale de Lille, Inria Lille - Nord Europe 2 LIFL - Laboratoire d-Informatique Fondamentale de Lille 3 French-Russian J-V Poncelet Laboratory

Abstract : BackgroundFrameshift mutations in protein-coding DNA sequences produce a drastic change in the resulting protein sequence, which prevents classic protein alignment methods from revealing the proteins- common origin. Moreover, when a large number of substitutions are additionally involved in the divergence, the homology detection becomes difficult even at the DNA level.ResultsWe developed a novel method to infer distant homology relations of two proteins, that accounts for frameshift and point mutations that may have affected the coding sequences. We design a dynamic programming alignment algorithm over memory-efficient graph representations of the complete set of putative DNA sequences of each protein, with the goal of determining the two putative DNA sequences which have the best scoring alignment under a powerful scoring system designed to reflect the most probable evolutionary process. Our implementation is freely available at http:-bioinfo.lifl.fr-path-.ConclusionsOur approach allows to uncover evolutionary information that is not captured by traditional alignment methods, which is confirmed by biologically significant examples.





Author: Marta Gîrdea - Laurent Noé - Gregory Kucherov -

Source: https://hal.archives-ouvertes.fr/



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