A glucose biosensor based on modified-enzyme incorporated within electropolymerised poly3,4-ethylenedioxythiophene PEDT filmsReport as inadecuate




A glucose biosensor based on modified-enzyme incorporated within electropolymerised poly3,4-ethylenedioxythiophene PEDT films - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 ITODYS UMR7086 - Interfaces, Traitements, Organisation et Dynamique des Systèmes 2 SPIN-ENSMSE - Centre Sciences des Processus Industriels et Naturels 3 P2MC-ENSMSE - Département Poudres et Matériaux Multi-Composants

Abstract : We have constructed and characterised a glucose sensor using glucose oxidase GOD covalently attached to carboxylic acid polyethyleneglycol PEG, called PEG–GOD. This modified enzyme was entrapped afterwards within poly3,4-ethylenedioxythiophene PEDT films electrogenerated on glassy carbon GC electrodes. The composite PEG–GOD-PEDT film is more porous than the film without enzyme PEDT+PEG. Data from electrochemical quartz microbalance ECQM and pH-stat experiments indicate a good relative activity of the modified enzyme, ca. 12–15%. Amperometric measurements, using ferrocenemethanol as the redox mediator, confirms that the modified enzyme is catalytically active. The effect of film thickness was also investigated. The sensitivities were quite similar for modified-GOD electrodes ca. 3 mA cm−2 M−1 and unmodified-GOD electrodes ca. 2.7 mA cm−2 M−1 but a better stability was obtained with modified PEG–GOD electrodes.

Keywords : Biosensors Covalent immobilisation Conducting polymers Glucose Electrochemical quartz microbalance





Author: Benoît Piro - Dang Lan Anh - Pham Minh Chau - Silvia Fabiano - Tran-Minh Canh -

Source: https://hal.archives-ouvertes.fr/



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