Effects of docosahexaenoic acid on some megakaryocytic cell gene expression of some enzymes controlling prostanoid synthesis.Report as inadecuate




Effects of docosahexaenoic acid on some megakaryocytic cell gene expression of some enzymes controlling prostanoid synthesis. - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 Régulations métaboliques, nutrition et diabètes

Abstract : Beneficial effects of docosahexaenoic acid DHA intake in the prevention of cardiovascular diseases are known, and platelets play a crucial role in cardiovascular complications. However, high doses of DHA may increase lipid peroxidation and induce deleterious effects, notably in platelets. This led us to investigate the effect of DHA on gene expression of some enzymes controlling redox status and prostanoid formation in human megakaryoblastic cells MEG-01 cell line. MEG-01 cells were incubated in presence of DHA 10 and 100 micromol-L for 6h. DHA enrichment up-regulated glutathione peroxidase-1 and thromboxane synthase mRNA. DHA increased gene catalase expression and up-regulated PPAR beta-delta and PPAR gamma mRNA in presence of high concentration of DHA. In conclusion, our results support an antioxidant mechanism of DHA. The effects of DHA on cellular redox status could, with others, provide an explanation for the beneficial influence of low consumption of DHA on cardiovascular events.

Keywords : docosahexaenoic acid gene expression megakaryocyte glutathione peroxidase catalase oxidative stress antioxidant thromboxane synthase phospholipids





Author: Nicolas Guillot - Cyrille Debard - Catherine Calzada - Hubert Vidal - Michel Lagarde - Evelyne Véricel -

Source: https://hal.archives-ouvertes.fr/



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