Optimized Extraction of Resveratrol from Arachis repens Handro by Ultrasound and Microwave: A Correlation Study with the Antioxidant Properties and Phenol ContentsReport as inadecuate




Optimized Extraction of Resveratrol from Arachis repens Handro by Ultrasound and Microwave: A Correlation Study with the Antioxidant Properties and Phenol Contents - Download this document for free, or read online. Document in PDF available to download.

The Scientific World Journal - Volume 2016 2016, Article ID 5890897, 10 pages -

Research Article

Laboratório de Micropropagação e Transformação de Plantas, Cellular Biology Department, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil

Departamento de Engenharia de Bioprocessos e Biotecnologia, Universidade Estadual Paulista UNESP, Campus Botucatu, 18610 307 Botucatu, SP, Brazil

Biocatalysis and Organic Synthesis Group, Chemistry Institute, Federal University of Rio de Janeiro, 22941-909 Rio de Janeiro, RJ, Brazil

Laboratório de Produtos Naturais e Ensaios Biológicos LaProNEB, Pharmacy Faculty, Department of Natural Products and Food, Federal University of Rio de Janeiro, 21.941-902 Rio de Janeiro, RJ, Brazil

Received 15 April 2016; Revised 2 August 2016; Accepted 31 August 2016

Academic Editor: Cristina Garcia-Viguera

Copyright © 2016 Leonardo Garcia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The vegetal species Arachis repens, commonly known as peanut grass, was studied and, for the first time, we detected the presence of the bioactive compound trans-resveratrol -RSV. We compared the efficiency of three different methodologies conventional maceration CM, ultrasound-assisted extractions UAE, and microwave-assisted extractions MAE concerning total phenolics TP and resveratrol -RSV content, followed by antioxidant activity AA evaluation. By CM, at 1 h, the highest RSV content  mg-L and, correspondingly, the highest DPPH capture % were found. The TP contents, at 1 h, presented the highest value  mg-g GAE. By the UAE, the maximum yields of TP 357.18 mg-g GAE and RSV 2.14 mg-L, as well as, the highest AA 70.95%, were obtained by 5 min after a maceration pretreatment, on the solid-solvent ratio 1 : 40 w-v. For MAE, a central composite rotatable design CCRD was applied followed by the FFD design in order to evaluate the statistical effects of four independent variables on the extraction of RSV. The optimal conditions established for obtaining the highest recovery 2.516 mg-g were 20 min; 90% MeOH aq.; 120 rpm; 60°C; and solid-solvent ratio: 1 : 35 w-v. Relevant correlations were established considering the TP and RSV contents, as well as the AA, corroborating obvious advantages of such techniques in terms of high extraction efficiency in shorter times.





Author: Leonardo Garcia, Renata Garcia, Georgia Pacheco, Felipe Sutili, Rodrigo De Souza, Elisabeth Mansur, and Ivana Leal

Source: https://www.hindawi.com/



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