In Vitro Bacterial Contamination of Amniotic Fluid: Effects on Fluorescence Polarization Lung Maturity TestingReport as inadecuate




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Infectious Diseases in Obstetrics and Gynecology - Volume 3 1995, Issue 3, Pages 98-101

Clinical Study

Department of Obstetrics and Gynecology, University of Colorado, Denver, CO, USA

Department of Clinical Pharmacology, University of Colorado, Denver, CO, USA

Department of Pathology, University of Utah School of Medicine, Salt Lake, UT, USA

Magee-Womens Research Institute, 204 Craft Avenue, Pittsburgh 15213, PA, USA

Received 22 May 1995; Accepted 25 July 1995

Copyright © 1995 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective: We sought to determine the effect of bacteria on fluorescence polarization FPOL testing of amniotic fluid.

Methods: Fusobacterium necrophorum and Escherichia coli were inoculated at concentrations of 103 and 106-ml in amniotic-fluid specimens from 4 patients with no clinical or laboratory evidence of infection. The FPOL results were obtained at inoculation and again at 24 h of incubation. The results were compared using analysis of variance ANOVA.

Results: The FPOL results from inoculated specimens were all within 2% of the uninoculated controls. The specimens incubated with bacteria showed a < 1–19% variation when compared with the time-zero uninoculated controls. However, uninoculated controls incubated for 24 h exhibited a 2–12% variation when compared with the time-zero controls, suggesting that the variation present was not secondary to the bacterial co-incubation.

Conclusions: In vitro, neither bacterial inoculation nor prolonged co-incubation influences FPOL results beyond the effect of incubation alone. FPOL appears to be an appropriate test to assess fetal lung maturity in patients in whom intraamniotic infection is a concern.





Author: R. Phillip Heine, Susan Harding, Pegi Emmett, Edward Ashwood, and Roger R. Lenke

Source: https://www.hindawi.com/



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