A Long-Acting BMP-2 Release System Based on Poly3-hydroxybutyrate Nanoparticles Modified by Amphiphilic Phospholipid for Osteogenic DifferentiationReportar como inadecuado




A Long-Acting BMP-2 Release System Based on Poly3-hydroxybutyrate Nanoparticles Modified by Amphiphilic Phospholipid for Osteogenic Differentiation - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BioMed Research International - Volume 2016 2016, Article ID 5878645, 10 pages -

Research ArticleDepartment of Orthopaedics, The Sixth Affiliated People’s Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai 200233, China

Received 14 March 2016; Accepted 17 May 2016

Academic Editor: Fang Sun

Copyright © 2016 Xiaochun Peng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We explored a novel poly3-hydroxybutyrate PHB nanoparticle loaded with hydrophilic recombinant human BMP-2 with amphiphilic phospholipid BPC-PHB NP for a rapid-acting and long-acting delivery system of BMP-2 for osteogenic differentiation. The BPC-PHB NPs were prepared by a solvent evaporation method and showed a spherical particle with a mean particle size of 253.4 nm, mean zeta potential of −22.42 mV, and high entrapment efficiency of 77.18%, respectively. For BPC-PHB NPs, a short initial burst release of BMP-2 from NPs in 24 h was found and it has steadily risen to reach about 80% in 20 days for in vitro test. BPC-PHB NPs significantly reduced the burst release of BMP-2, as compared to that of PHB NPs loading BMP-2 without PL B-PHB NPs. BPC-PHB NPs maintained the content of BMP-2 for a long-term osteogenic differentiation. The OCT-1 cells with BPC-PHB NPs have high ALP activity in comparison with others. The gene markers for osteogenic differentiation were significantly upregulated for sample with BPC-PHB NPs, implying that BPC-PHB NPs can be used as a rapid-acting and long-acting BMP-2 delivery system for osteogenic differentiation.





Autor: Xiaochun Peng, Yunsu Chen, Yamin Li, Yiming Wang, and Xianlong Zhang

Fuente: https://www.hindawi.com/



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