Solution structure of the N-terminal dsRBD of Drosophila ADAR and interaction studies with RNA.Reportar como inadecuado

Solution structure of the N-terminal dsRBD of Drosophila ADAR and interaction studies with RNA. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 IMBB - Institute for Molecular Biology and Biophysics 2 MRC - MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine

Abstract : Adenosine deaminases that act on RNA ADAR catalyze adenosine to inosine A-to-I editing in double-stranded RNA dsRNA substrates. Inosine is read as guanosine by the translation machinery; therefore A-to-I editing events in coding sequences may result in recoding genetic information. Whereas vertebrates have two catalytically active enzymes, namely ADAR1 and ADAR2, Drosophila has a single ADAR protein dADAR related to ADAR2. The structural determinants controlling substrate recognition and editing of a specific adenosine within dsRNA substrates are only partially understood. Here, we report the solution structure of the N-terminal dsRNA binding domain dsRBD of dADAR and use NMR chemical shift perturbations to identify the protein surface involved in RNA binding. Additionally, we show that Drosophila ADAR edits the R-G site in the mammalian GluR-2 pre-mRNA which is naturally modified by both ADAR1 and ADAR2. We then constructed a model showing how dADAR dsRBD1 binds to the GluR-2 R-G stem-loop. This model revealed that most side chains interacting with the RNA sugar-phosphate backbone need only small displacement to adapt for dsRNA binding and are thus ready to bind to their dsRNA target. It also predicts that dADAR dsRBD1 would bind to dsRNA with less sequence specificity than dsRBDs of ADAR2. Altogether, this study gives new insights into dsRNA substrate recognition by Drosophila ADAR.

keyword : RNA editing ADAR Drosophila NMR dsRBD dsRBM dsRNA structure

Autor: Pierre Barraud - Bret S E Heale - Mary A O-Connell - Frédéric H-T Allain -



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