Assessment of DNA Damage and Telomerase Activity in Exfoliated Urinary Cells as Sensitive and Noninvasive Biomarkers for Early Diagnosis of Bladder Cancer in Ex-Workers of a Rubber Tyres IndustryReportar como inadecuado




Assessment of DNA Damage and Telomerase Activity in Exfoliated Urinary Cells as Sensitive and Noninvasive Biomarkers for Early Diagnosis of Bladder Cancer in Ex-Workers of a Rubber Tyres Industry - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BioMed Research International - Volume 2014 2014, Article ID 370907, 8 pages -

Research Article

Department of Occupational Medicine, INAIL-Italian Workers’ Compensation Authority, Research Area, Monteporzio Catone, 00040 Rome, Italy

Bioscience Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori, Meldola, 47014 Forlì-Cesena, Italy

Department of Traumatology, Orthopaedics and Occupational Medicine, University of Turin, 10126 Turin, Italy

Received 15 November 2013; Accepted 22 March 2014; Published 30 April 2014

Academic Editor: Ahmed El Nemr

Copyright © 2014 Delia Cavallo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of the present study was to identify sensitive and noninvasive biomarkers of early carcinogenic effect at target organ to use in biomonitoring studies of workers at risk for previous occupational exposure to potential carcinogens. Standard urine cytology Papanicolaou staining test, comet assay, and quantitative telomerase repeat amplification protocol TRAP assay were performed in 159 ex-rubber workers employed in tyres production and 97 unexposed subjects. In TRAP positive cases, a second level analysis using FISH Urovysion was done. Cystoscopy results were available for 11 individuals whose 6 FISH-TRAP-comet positive showed in 3 cases a dysplastic condition confirmed by biopsy, 1 comet positive resulted in infiltrating UBC to the biopsy and with hyperplasia and slight dysplasia to the urinary cytology, 1 comet positive resulted in papillary superficial UBC to the biopsy, 1 FISH-TRAP positive showed a normal condition, and 2 TRAP positive showed in one case a phlogosis condition. The results evidenced good concordance of TRAP, comet, and FISH assays as early biomarkers of procarcinogenic effect confirmed by the dysplastic condition and UBC found by cystoscopy-biopsy analysis. The analysis of these markers in urine cells could be potentially more accurate than conventional cytology in monitoring workers exposed to mixture of bladder potential carcinogens.





Autor: Delia Cavallo, Valentina Casadio, Sara Bravaccini, Sergio Iavicoli, Enrico Pira, Canzio Romano, Anna Maria Fresegna, Raffae

Fuente: https://www.hindawi.com/



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