Determination of Free Levels of Cinitipride in Human Plasma by Liquid Chromatography-Tandem Mass SpectrometryReportar como inadecuado




Determination of Free Levels of Cinitipride in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

E-Journal of Chemistry - Volume 5 2008, Issue 3, Pages 453-460

Department of Chemistry, G.N.Khalsa College, Matunga, Mumbai-400 019, India

Received 5 October 2007; Accepted 20 December 2007

Copyright © 2008 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A rapid, sensitive and specific method to quantify cinitapride in human plasma using risperidone as the internal standard is described. Sample preparation involved simple solid phase extraction procedure. The extract wasanalyzed by high performance liquid chromatography coupled to electrospray tandem mass spectrometry API-4000 LC-MS-MS. Chromatography was performed isocratically on Thermo Hypurity C18 analytical column, 50 mm x4.6 mm, 5 µm i.d

The assay of cinitapride was linear calibration curve over the range 20.118 pg mL

to 2011.797 pg mL

. Plasma concentrations of cinitapride were determined by LC-MS-MS with a limit of quantification of 20.118 pg mL

that allowed an appropriate characterization of the pharmacokinetic profile of cinitapride at the therapeutic dose. The method was successfully applied to the bioequivalence study of cinitapride tablet 1.0 mg administered as a single oral dose.





Autor: Shikha M. N. Roy, Santos H. M. Yetal, Sangita V. Chavan, Vara D. R. Pradhan, and Santosh S. Joshi

Fuente: https://www.hindawi.com/



DESCARGAR PDF




Documentos relacionados