Diverse Reductive Dehalogenases Are Associated with Clostridiales-Enriched Microcosms Dechlorinating 1,2-DichloroethaneReportar como inadecuado




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BioMed Research International - Volume 2015 2015, Article ID 242856, 11 pages -

Research Article

Department of Food, Environmental and Nutritional Sciences DeFENS, University of Milan, 20133 Milan, Italy

Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia

Laboratory for Microbial Ecology and Technology LabMET, Ghent University, 9000 Ghent, Belgium

Research Center for Non-Conventional Energy, Istituto Eni Donegani, Environmental Technologies, 20097 San Donato Milanese, Italy

Received 8 November 2014; Accepted 6 March 2015

Academic Editor: Ameur Cherif

Copyright © 2015 Giuseppe Merlino et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The achievement of successful biostimulation of active microbiomes for the cleanup of a polluted site is strictly dependent on the knowledge of the key microorganisms equipped with the relevant catabolic genes responsible for the degradation process. In this work, we present the characterization of the bacterial community developed in anaerobic microcosms after biostimulation with the electron donor lactate of groundwater polluted with 1,2-dichloroethane 1,2-DCA. Through a multilevel analysis, we have assessed i the structural analysis of the bacterial community; ii the identification of putative dehalorespiring bacteria; iii the characterization of functional genes encoding for putative 1,2-DCA reductive dehalogenases RDs. Following the biostimulation treatment, the structure of the bacterial community underwent a notable change of the main phylotypes, with the enrichment of representatives of the order Clostridiales. Through PCR targeting conserved regions within known RD genes, four novel variants of RDs previously associated with the reductive dechlorination of 1,2-DCA were identified in the metagenome of the Clostridiales-dominated bacterial community.





Autor: Giuseppe Merlino, Annalisa Balloi, Massimo Marzorati, Francesca Mapelli, Aurora Rizzi, Davide Lavazza, Francesca de Ferra, 

Fuente: https://www.hindawi.com/



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