A role for the microtubule end protein Bik1 CLIP170 and the Rho1 GTPase in Snc1 traffickingReportar como inadecuado

A role for the microtubule end protein Bik1 CLIP170 and the Rho1 GTPase in Snc1 trafficking - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 GIN Grenoble Institut des Neurosciences 2 Institut de Biosciences et Biotechnologies de Grenoble BIG - CEA Sciences 3 Department of Biology Zürich, Switzerland 4 DIG CANCER - Dynamique de l-information génétique : bases fondamentales et cancer 5 BGE - UMR S1038 - Laboratoire de Biologie à Grande Échelle

Abstract : The diversity of microtubule functions is dependent on the status of tubulin C-termini. To address the physiological role of the C-terminal aromatic residue of -tubulin, a tub1-Glu yeast strain expressing an -tubulin devoid of its C-terminal amino-acid was used to perform a genome-wide-lethality screen. The identified synthetic lethal genes suggested links with endocytosis and related processes. In the tub1-Glu strain, the routing of the v-SNARE Snc1 was strongly impaired, with a loss of its polarized distribution in the bud and Abp1, an actin patch-endocytic marker, developed comet-tails structures. Snc1 trafficking necessitated dynamic microtubules but not dynein and kinesin motors. Interestingly, deletion of the microtubule +end protein Bik1 CLIP170, which is preferentially recruited to the C-terminal residue of -tubulin, similarly resulted in Snc1 trafficking defects. Finally, constitutively active Rho1 rescued both Bik1 localization at microtubule +ends in tub1-Glu strain and a correct Snc1 trafficking in a Bik1-dependent manner. Our results provide the first evidence for a role of microtubule +ends in membrane-cargo trafficking in yeast, through Rho1- and Bik1-dependent mechanisms and highlight the importance of -tubulin last amino-acid in this process.

Keywords : trafficking +Tips tyrosination Microtubules CLIP170 Rho1

Autor: Cécile Boscheron - Fabrice Caudron - Sophie Loeillet - Charlotte Peloso - Marine Mugnier - Laetitia Kurzawa - Alain Nicolas - Er

Fuente: https://hal.archives-ouvertes.fr/


Documentos relacionados