Exposure of fathead minnows to municipal wastewater effluent affects intracellular signaling pathways in the liverReportar como inadecuado

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* Corresponding author 1 INRS-IAF - Institut Armand Frappier 2 Fluvial Ecosystems Research, Aquatic Ecosystems Research Protection Branch

Abstract : Municipal wastewater effluent can impact its receiving environment. In the St. Lawrence River, male fish livingdownstream from Montreal exhibit increased hepatic vitellogenin, intersex, delayed spermatogenesis andaltered immune function. Few studies have examined genome-wide effects associated with municipal effluentexposure in fish to decipher the mechanisms of toxicity. The present objective was to identify hepatic cellular signalingpathways in fathead minnows following exposure to municipal wastewater effluent. Immature minnowswere exposed for 21 days to either 0% Control or 20% municipal effluent, the highest concentration in the St.Lawrence River. Hepatic RNA was extracted and used to hybridize a fathead minnow oligonucleotide microarraycontaining approximately 15 k gene sequences. A total of 1300 genes were differentially expressed, of which 309genes had more than 2-fold change in expression level between control and MWWE-exposed fish. Of those, 118were up-regulated and 191 were down-regulated. Altered genes grouped according to function, indicated effectson various signaling pathways, apoptosis, immune responses, and cellular metabolism. Pathway analysis softwarepredicted at least 5 signaling pathways that were altered by treatment: cell adhesion, inflammation, variouskinases, estrogen receptor signaling and WNT signaling. Various components of the canonicalWnt pathway weredramatically down-regulated, while several other genes involved in the non-canonical Wnt pathway, such asWnt4, LRP6, and PPP2R5E, which are known to inhibit the canonical Wnt pathway, were increased. These resultsindicate that municipal wastewater effluent from Montreal can target and inhibit various signaling includingthose implicated in hepatic Wnt signaling pathway in fathead minnows

Autor: Jennifer Arstikaitis - F. Gagné - Daniel G Cyr -

Fuente: https://hal.archives-ouvertes.fr/


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