Downmodulation of Vaccine-Induced Immunity and Protection against the Intracellular Bacterium Francisella tularensis by the Inhibitory Receptor FcγRIIBReportar como inadecuado




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Journal of Immunology Research - Volume 2015 2015, Article ID 840842, 13 pages -

Research Article

Center for Immunology and Microbial Disease, Albany Medical College, Albany, NY 12208, USA

Regeneron Pharmaceuticals, 81 Columbia Turnpike, Rensselaer, NY 12144, USA

Department of Biological Sciences, Seton Hall University, South Orange, NJ 07079, USA

Regeneron Pharmaceuticals, 777 Old Saw Mill River Road, Tarrytown, NY 10591, USA

Pfizer, 610 Main Street, Cambridge, MA 02139, USA

Received 25 September 2014; Revised 5 January 2015; Accepted 6 January 2015

Academic Editor: Oscar Bottasso

Copyright © 2015 Brian J. Franz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Fc gamma receptor IIB FcγRIIB is the only Fc gamma receptor FcγR which negatively regulates the immune response, when engaged by antigen- Ag- antibody Ab complexes. Thus, the generation of Ag-specific IgG in response to infection or immunization has the potential to downmodulate immune protection against infection. Therefore, we sought to determine the impact of FcγRIIB on immune protection against Francisella tularensis Ft, a Category A biothreat agent. We utilized inactivated Ft iFt as an immunogen. Naïve and iFt-immunized FcγRIIB knockout KO or wildtype WT mice were challenged with Ft-live vaccine strain LVS. While no significant difference in survival between naïve FcγRIIB KO versus WT mice was observed, iFt-immunized FcγRIIB KO mice were significantly better protected than iFt-immunized WT mice. Ft-specific IgA in serum and bronchial alveolar lavage, as well as IFN-γ, IL-10, and TNF-α production by splenocytes harvested from iFt-immunized FcγRIIB KO, were also significantly elevated. In addition, iFt-immunized FcγRIIB KO mice exhibited a reduction in proinflammatory cytokine levels in vivo at 5 days after challenge, which correlates with increased survival following Ft-LVS challenge in published studies. Thus, these studies demonstrate for the first time the ability of FcγRIIB to regulate vaccine-induced IgA production and downmodulate immunity and protection. The immune mechanisms behind the above observations and their potential impact on vaccine development are discussed.





Autor: Brian J. Franz, Ying Li, Constantine Bitsaktsis, Bibiana V. Iglesias, Giang Pham, Raju Sunagar, Sudeep Kumar, and Edmund J.

Fuente: https://www.hindawi.com/



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