Peptide binding to ochratoxin A mycotoxin: a new approach in conception of biosensors.Reportar como inadecuado

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* Corresponding author 1 EMA - École des Mines d-Alès 2 HLA-DR - Gènes HLA-DR, Autoanticorps et Microchimérisme dans la Polyarthrite Rhumatoïde et la Sclérodermie 3 INSERM U836, équipe 3, Canaux calciques, fonctions et pathologies GIN - Grenoble Institut des Neurosciences

Abstract : Ochratoxin A OTA is a widespread and abundant natural carcinogenic mycotoxin produced by several species of Aspergillus and Penicillium fungi. Due to the ubiquitous presence of these fungi in food and potential risk for human health, a rapid and sensitive in vitro detection assay is required. Analytical methods for OTA detection-identification are generally based on liquid-liquid extraction, clean-up using an immunoaffinity column IAC, and identification by reversed-phase high pressure liquid chromatography with fluorescence detection HPLC-FLD. However, IACs are costly and have a short lifespan. Therefore, an interesting approach would appear to be the design and chemical synthesis of a mimotope peptide simulating mycotoxin-specific antibodies. We have developed a promising alternative method that is based on the use of peptides which are able to bind to specific chemical functions and-or molecular structures. Accordingly, a number of peptides derived from the structures of major redox proteins were selected and produced by chemical solid phase syntheses. The ability of such peptides to bind to ochratoxin A was evaluated by HPLC. The peptide NF04 structurally derived from an oxidoreductase enzyme, which was found to be the sole potently reactive compound among tested molecules, was further evaluated in a peptide-based enzyme-linked immunosorbent assay peptide-based ELISA, thus confirming its specific interaction with ochratoxin A.

Keywords : peptide binding peptide-based assay ochratoxin A mycotoxin

Autor: Ingrid Bazin - Nicolas Andreotti - Aziza Ibn Hadj Hassine - Michel De Waard - Jean-Marc Sabatier - Catherine Gonzalez -



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