Simple control of fed-batch processes for recombinant protein production withReport as inadecuate

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* Corresponding author 1 Institute of Biochemistry and Biotechnology 2 Institute of Automation and Control Systems

Abstract : A very simple but effective process control technique is proposed that leads to a high batch-to-batch reproducibility with respect to biomass concentration as well as the specific biomass growth rate profiles in fermentations performed during recombinant protein production. It makes use of the well-established temperature controllers in currently used fermenters, but takes its information from the difference between the controlled culture temperature and the temperature of the coolant fed to the fermenter-s cooling jacket as adjusted by the fermenter temperature controller. For process control purposes this measured difference is corrected regarding stirrer influences and cumulated before it is used as a new process control variable. As a spin-off of this control, it becomes possible to estimate online the oxygen mass transfer rates and the corresponding values during the real cultivation process.

Keywords : Fed-batch Heat flow High cell density cultivation Protein production

Author: Sebastian Schaepe - Artur Kuprijanov - Mathias Aehle - Rimvydas Simutis - Andreas Lübbert -



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