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* Corresponding author 1 Leibniz Institute of Polymer Research Dresden 2 BASF SE

Abstract : The effect of the activity of immobilized enzymes on the initial attachment of pathogenic bacteria commonly associated with nosocomial infections and was investigated. The proteolytic enzymes, subtilisin A and the glycoside hydrolase cellulose, were covalently attached onto polyethylene-maleic anhydride copolymer films. A comparison between active and heat-inactivated surfaces showed that while the activity of immobilized cellulase reduced the attachment of by 67%, it had no effect on the attachment of . Immobilized subtilisin A had opposite effects: the active enzyme had no effect on the attachment of but reduced the attachment of by 44%. The results suggest that different biomolecules are involved in the initial steps of attachment of different bacteria, and that the development of broad-spectrum antifouling enzymatic coatings will need to involve the co-immobilization of enzymes.

keyword : Bioactive surfaces Biofilm Cellulase Subtilisin

Autor: Ana L. Cordeiro - Catharina Hippius - Carsten Werner -



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