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* Corresponding author 1 Institut d-oncologie-développement Albert Bonniot de Grenoble 2 IBCP - Institut de biologie et chimie des protéines Lyon 3 ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires 4 Physiopathologie moléculaire et nouveaux traitements des hépatites virales 5 SPRAM - UMR 5819 - Structures et propriétés d-architectures moléculaire 6 INSERM U823, équipe 8 Immunologie Analytique des Pathologies Chroniques Institut d-oncologie-développement Albert Bonniot de Grenoble

Abstract : Biosensors in microarray format provide promising tools for high-throughput analyses of complex samples. Although they are able to detect, quantify and characterize a multitude of compounds, most of the available devices are specialized in the analysis of one type of interaction, limiting their application to a define area. The aim of our work was to develop and characterize versatile protein or peptide microarrays suitable for the simultaneous analysis of a large panel of biological interactions. Our system involved a simple procedure to immobilized proteins or peptides, based on pyrrole electropolymerization, and ligand binding was detected by imaging the surface plasmon resonance. We demonstrated its suitability in three different contexts, i.e. humoral response characterization, ion binding analysis and cell detection. This work evidences the potentiality of this approach which allows multiparametric, high-throughput and label-free analysis of biological samples suitable for the detection of compounds as various as proteins, ions or cells and the characterization of their interaction with peptides or proteins.

Keywords : Microarray Surface Plasmon Resonance imaging protein peptide cell ion





Autor: Marie-Bernadette Villiers - Sandra Cortes - Carine Brakha - Jean-Pierre Lavergne - Christophe Marquette - Paul Deny - Thierry Liv

Fuente: https://hal.archives-ouvertes.fr/



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