Polymer adhesive surface as flexible generic platform for multiplexed assays biochip production.Report as inadecuate

Polymer adhesive surface as flexible generic platform for multiplexed assays biochip production. - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires 2 Axo S - Axo Science 3 Hypoxie et physiopathologies cardiovasculaire et respiratoire 4 INSERM U823, équipe 8 Immunologie Analytique des Pathologies Chroniques Institut d-oncologie-développement Albert Bonniot de Grenoble

Abstract : The present report describes the integration and application possibilities of a new microarray concept based on adhesive surface. The method was shown to enable the straightforward production of 384 and 1536-well plates modified with 100 and 25 spots per well, respectively. Such in-well densities were only possible thanks to the fabrication process which implies first the deposition of the microarray on a flat adhesive surface and then its assembly with bottomless 384 or 1536-well plates. The concept was also confronted to various applications such as oligonucleotide detection, localised cell culture onto spotted adhesion proteins and immobilisation of peptide or active antibodies for immunoassays. In the particular case of immunotesting, the study focused on liver diseases diagnosis and more particularly on the detection of either one liver cancer marker, the alpha-fetoprotein, or the detection of Hepatitis C Virus infection. In every cases, interesting performances were obtained directly in crude patient serum, proof of the robust and generic aspect of the platform.

Keywords : Adhesive surface AFP Colorimetry HCV Localised cell culture Microarray Multiplexed assays

Author: Céline Mandon - Ophélie Berthuy - Benjamin Corgier - Gaelle Le Goff - Patrice Faure - Patrice Marche - Loic Blum - Christophe M

Source: https://hal.archives-ouvertes.fr/


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