Elucidation of Danzhixiaoyao Wan and Its Constituent Herbs on Antioxidant Activity and Inhibition of Nitric Oxide ProductionReport as inadecuate

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Evidence-Based Complementary and Alternative Medicine - Volume 4 2007, Issue 4, Pages 425-430

Original Article

Centre for Phytochemistry and Pharmacology, Southern Cross University, Lismore NSW 2480, Australia

Department of Pharmacy, Shanxi Provincial People's Hospital, 99 Shuangtasi Street, Taiyuan, Shanxi 030012, China

Received 10 April 2006; Accepted 13 October 2006

Copyright © 2007 Hui Liao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Danzhixiaoyao Wan DW is a common 10 herbs formulation in China for regulating several clinical conditions affecting women. This research tried to explain one of DW-s functions, purging heat, using in vitro pharmacological analyses. The whole formulation and each single herb of DW were compared based on antioxidant activity with the oxygen radical absorbance capacity ORAC assay, and for their inhibitory effect IE on nitric oxide NO production by lipopolysaccharide LPS-activated RAW 264.7 macrophages with the Griess assay. The results showed that DW as a whole formulation had both antioxidant activity and an IE on NO production, while the individual herb component of DW varied in their ORAC values and inhibition of NO production. The ORAC value of the whole DW was 450 μmol TE g

. The order of antioxidant ORAC activity of the single herbs was: Mentha haplocalyx 1352 μmol TE g

> Glycyrrhiza uralensis 1184 μmol TE g

> Gardenia jasminoides 1129 μmol TE g

> Paeonia suffruticosa 465 μmol TE g

, with the contributions being additive rather than synergistic. The production of nitrite by stimulated RAW 264.7 murine macrophages unstimulated: 0.5 ± 0.1 μM versus LPS: 38.9 ± 2.3 μM was significantly inhibited P < 0.05 by M. haplocalyx, G. jasminoides, Bupleurum chinense and Paeonia lactiflora. DW as a whole had an IE on NO production, but this was not significant. The single herb M. haplocalyx had the highest ORAC value and the highest IE on NO production, followed by G. jasminoides. Both of these herbs have the ‘purging heat’ property in the theory of traditional Chinese medicine and this property of the samples may be correlated with the antioxidant activity and IE on NO production.

Author: Hui Liao, Linda K. Banbury, and David N. Leach

Source: https://www.hindawi.com/


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