In Vitro Behavior of Human Adipose Tissue-Derived Stem Cells on Polyε-caprolactone Film for Bone Tissue Engineering ApplicationsReportar como inadecuado

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BioMed Research International - Volume 2015 2015, Article ID 323571, 12 pages -

Research Article

Department of Surgery and Translational Medicine, University of Florence, 50139 Florence, Italy

BIOLab Research Group, Department of Chemistry and Industrial Chemistry, University of Pisa, UdR INSTM Pisa, 56124 Pisa, Italy

Microdentistry Institute, 50123 Florence, Italy

Received 22 May 2015; Accepted 10 September 2015

Academic Editor: Giuseppe Cama

Copyright © 2015 Cecilia Romagnoli et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Bone tissue engineering is an emerging field, representing one of the most exciting challenges for scientists and clinicians. The possibility of combining mesenchymal stem cells and scaffolds to create engineered tissues has brought attention to a large variety of biomaterials in combination with osteoprogenitor cells able to promote and regenerate bone tissue. Human adipose tissue is officially recognized as an easily accessible source of mesenchymal stem cells AMSCs, a significant factor for use in tissue regenerative medicine. In this study, we analyze the behavior of a clonal finite cell line derived from human adipose tissue seeded on polyε-caprolactone PCL film, prepared by solvent casting. PCL polymer is chosen for its good biocompatibility, biodegradability, and mechanical properties. We observe that AMSCs are able to adhere to the biomaterial and remain viable for the entire experimental period. Moreover, we show that the proliferation process and osteogenic activity of AMSCs are maintained on the biofilm, demonstrating that the selected biomaterial ensures cell colonization and the development of an extracellular mineralized matrix. The results of this study highlight that AMSCs and PCL film can be used as a suitable model to support regeneration of new bone for future tissue engineering strategies.

Autor: Cecilia Romagnoli, Roberto Zonefrati, Gianna Galli, Dario Puppi, Alessandro Pirosa, Federica Chiellini, Francesco Saverio Ma



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