Quercetin and Quercetin-Rich Red Onion Extract Alter Pgc-1α Promoter Methylation and Splice Variant ExpressionReportar como inadecuado

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PPAR Research - Volume 2017 2017, Article ID 3235693, 8 pages - https:-doi.org-10.1155-2017-3235693

Research ArticleDepartment of Nutrition Science, Purdue University, West Lafayette, IN, USA

Correspondence should be addressed to Tara M. Henagan

Received 27 July 2016; Revised 6 November 2016; Accepted 18 December 2016; Published 16 January 2017

Academic Editor: Rozalyn M. Anderson

Copyright © 2017 Prasad P. Devarshi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Pgc-1α and its various isoforms may play a role in determining skeletal muscle mitochondrial adaptations in response to diet. 8 wks of dietary supplementation with the flavonoid quercetin Q or red onion extract ROE in a high fat diet HFD ameliorates HFD-induced obesity and insulin resistance in C57BL-J mice while upregulating Pgc-1α and increasing skeletal muscle mitochondrial number and function. Here, mice were fed a low fat LF, high fat HF, high fat plus quercetin HF + Q, or high fat plus red onion extract HF + RO diet for 9 wks and skeletal muscle Pgc-1α isoform expression and DNA methylation were determined. Quantification of various Pgc-1α isoforms, including isoforms Pgc-1α-a, Pgc-1α-b, Pgc-1α-c, Pgc-1α4, total NT-Pgc-1α, and FL-Pgc-1α, showed that only total NT-Pgc-1α expression was increased in LF, HF + Q, and HF + RO compared to HF. Furthermore, Q supplementation decreased Pgc-1α-a expression compared to LF and HF, and ROE decreased Pgc-1α-a expression compared to LF. FL-Pgc-1α was decreased in HF + Q and HF + RO compared to LF and HF. HF exhibited hypermethylation at the −260 nucleotide nt in the Pgc-1α promoter. Q and ROE prevented HFD-induced hypermethylation. −260 nt methylation levels were associated with NT-Pgc-1α expression only. Pgc-1α isoform expression may be epigenetically regulated by Q and ROE through DNA methylation.

Autor: Prasad P. Devarshi, Aarin D. Jones, Erin M. Taylor, Barbara Stefanska, and Tara M. Henagan

Fuente: https://www.hindawi.com/


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