Minimum antibiotic levels for selecting a resistance plasmid in a gnotobiotic animal model.Reportar como inadecuado

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* Corresponding author 1 Xénobiotiques 2 LGC - Laboratoire de Génétique Cellulaire

Abstract : The minimum antibiotic concentrations for selecting an R plasmid in vivo were determined in germfree mice colonized by two isogenic strains of Escherichia coli, one of which carried an R plasmid. Seventy groups of three gnotobiotic mice were given low doses of ampicillin, colistin, flumequin, gentamicin, tetracycline, or streptomycin via drinking water for 2 weeks. The equilibrium between susceptible and resistant populations of bacteria was monitored daily in feces and compared with that of control mice given pure water. This model yielded reproducible data, and dose and response were strongly correlated. The minimum selecting doses ranged from 0.9 to 12.8 micrograms-ml of water, depending on the antibiotic and the R plasmid. The use of mathematical models and complementary in vitro experiments accounted for the effect of the low antibiotic levels.

Keywords : Antimicrobial Agents minimum antibiotic concentration selection R plasmid in vivo germfree mouse mice Escherichia coli gnotobiotic mice ampicillin colistin flumequin gentamicin tetracycline streptomycin resistant bacteria animal model mathematical model minimum selecting concentration residues food risk assessment veterinary treatment

Autor: D. E. Corpet - S. Lumeau - F. Corpet -



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