Antiviral Activity of Total Flavonoid Extracts from Selaginella moellendorffii Hieron against Coxsackie Virus B3 In Vitro and In VivoReport as inadecuate




Antiviral Activity of Total Flavonoid Extracts from Selaginella moellendorffii Hieron against Coxsackie Virus B3 In Vitro and In Vivo - Download this document for free, or read online. Document in PDF available to download.

Evidence-Based Complementary and Alternative MedicineVolume 2014 2014, Article ID 950817, 7 pages

Research Article

Key Laboratory of TCM Resource and TCM Compound Co-Constructed by Hubei Province and Ministry of Education, Hubei University of Traditional Chinese Medicine, Wuhan 430065, China

Wuhan Bioengineering Institute, Wuhan 430415, China

State Key Laboratory of Virology, School of Medicine, Institute of Medical Virology, Wuhan University, Wuhan 430071, China

Received 11 February 2014; Revised 22 April 2014; Accepted 30 April 2014; Published 19 May 2014

Academic Editor: Shi-Biao Wu

Copyright © 2014 Dan Yin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The antiviral activity of total flavonoid extracts from Selaginella moellendorffii Hieron and its main constituents amentoflavone were investigated against coxsackie virus B3 CVB3. When added during or after viral infection, the extracts and amentoflavone prevented the cytopathic effect CPE of CVB3, as demonstrated in a 3-4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide MTT colorimetric assay, with a 50% inhibitory concentration IC50 from to  μg-mL and to  μg-mL, respectively. KM mice were used as animal models to test the extracts- activity in vivo. Oral administration of the total flavonoid extracts at 300 mg-kg-day significantly reduced mean viral titers in the heart and kidneys as well as mortality after infection for 15 days. The experimental results demonstrate that in vitro and in vivo the model mice infected with CVB3 can be effectively treated by the total flavonoid extracts from Selaginella moellendorffii Hieron.





Author: Dan Yin, Juan Li, Xiang Lei, Yimei Liu, Zhanqiu Yang, and Keli Chen

Source: https://www.hindawi.com/



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