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BioMed Research International - Volume 2014 2014, Article ID 916521, 10 pages -

Research Article

Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas ENCB, Instituto Politecnico Nacional IPN, 11340 México City, DF, Mexico

Departamento de Inmunobioquímica, Torre de Investigación, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes INPer, Montes Urales 800, Colonia Lomas de Virreyes, 11000 México City, DF, Mexico

Departamento de Inmunologia, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional IPN, 11340 México City, DF, Mexico

Departamento of Biomedicina Molecular, Centro de Investigacion y de Estudios Avanzados CINVESTAV, IPN, 07360 México City, DF, Mexico

Received 5 February 2014; Revised 10 April 2014; Accepted 30 April 2014; Published 18 May 2014

Academic Editor: Angel Cataldi

Copyright © 2014 A. Cecilia Helguera-Repetto et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nontuberculous mycobacteria NTM have recently been recognized as important species that cause disease even in immunocompetent individuals. The mechanisms that these species use to infect and persist inside macrophages are not well characterised. To gain insight concerning this process we used THP-1 macrophages infected with M. abscessus, M. fortuitum, M. celatum, and M. tuberculosis. Our results showed that slow-growing mycobacteria gained entrance into these cells with more efficiency than fast-growing mycobacteria. We have also demonstrated that viable slow-growing M. celatum persisted inside macrophages without causing cell damage and without inducing reactive oxygen species ROS, as M. tuberculosis caused. In contrast, fast-growing mycobacteria destroyed the cells and induced high levels of ROS. Additionally, the macrophage cytokine pattern induced by M. celatum was different from the one induced by either M. tuberculosis or fast-growing mycobacteria. Our results also suggest that, in some cases, the intracellular survival of mycobacteria and the immune response that they induce in macrophages could be related to their growth rate. In addition, the modulation of macrophage cytokine production, caused by M. celatum, might be a novel immune-evasion strategy used to survive inside macrophages that is different from the one reported for M. tuberculosis.





Author: A. Cecilia Helguera-Repetto, Rommel Chacon-Salinas, Jorge F. Cerna-Cortes, Sandra Rivera-Gutierrez, Vianney Ortiz-Navarrete, 

Source: https://www.hindawi.com/



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