Murine norovirus infection does not cause major disruptions in the murine intestinal microbiotaReportar como inadecuado

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, 1:7

First Online: 18 February 2013Received: 22 August 2012Accepted: 09 January 2013


BackgroundMurine norovirus MNV is the most common gastrointestinal pathogen of research mice and can alter research outcomes in biomedical mouse models of inflammatory bowel disease IBD. Despite indications that an altered microbiota is a risk factor for IBD, the response of the murine intestinal microbiota to MNV infection has not been examined. Microbiota disruption caused by MNV infection could introduce the confounding effects observed in research experiments. Therefore, this study investigated the effects of MNV infection on the intestinal microbiota of wild-type mice.

ResultsThe composition of the intestinal microbiota was assessed over time in both outbred Swiss Webster and inbred C57BL-6 mice following MNV infection. Mice were infected with both persistent and non-persistent MNV strains and tissue-associated or fecal-associated microbiota was analyzed by 16S rRNA-encoding gene pyrosequencing. Analysis of intestinal bacterial communities in infected mice at the phylum and family level showed no major differences to uninfected controls, both in tissue-associated samples and feces, and also over time following infection, demonstrating that the intestinal microbiota of wild-type mice is highly resistant to disruption following MNV infection.

ConclusionsThis is the first study to describe the intestinal microbiota following MNV infection and demonstrates that acute or persistent MNV infection is not associated with major disruptions of microbial communities in Swiss Webster and C57BL-6 mice.

KeywordsMicrobiome Murine norovirus Pyrosequencing Electronic supplementary materialThe online version of this article doi:10.1186-2049-2618-1-7 contains supplementary material, which is available to authorized users.

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Autor: Adam M Nelson - Michael D Elftman - Amelia K Pinto - Megan Baldridge - Patrick Hooper - Justin Kuczynski - Joseph F Pet


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