The S-Layer Glycoprotein of the Crenarchaeote Sulfolobus acidocaldarius Is Glycosylated at Multiple Sites with Chitobiose-Linked N-GlycansReportar como inadecuado




The S-Layer Glycoprotein of the Crenarchaeote Sulfolobus acidocaldarius Is Glycosylated at Multiple Sites with Chitobiose-Linked N-Glycans - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

ArchaeaVolume 2010 2010, Article ID 754101, 10 pages

Research Article

Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK

Molecular Biology of Archaea, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Straße 10, 35043 Marburg, Germany

Centre for Integrative Systems Biology, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK

Received 14 May 2010; Accepted 26 July 2010

Academic Editor: Jerry Eichler

Copyright © 2010 Elham Peyfoon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Glycosylation of the S-layer of the crenarchaea Sulfolobus acidocaldarius has been investigated using glycoproteomic methodologies. The mature protein is predicted to contain 31 N-glycosylation consensus sites with approximately one third being found in the C-terminal domain spanning residues - . Since this domain is rich in Lys and Arg and therefore relatively tractable to glycoproteomic analysis, this study has focused on mapping its N-glycosylation. Our analysis identified nine of the 11 consensus sequence sites, and all were found to be glycosylated. This constitutes a remarkably high glycosylation density in the C-terminal domain averaging one site for each stretch of 30–40 residues. Each of the glycosylation sites observed was shown to be modified with a heterogeneous family of glycans, with the largest having a composition Gl Ma GlcNA plus 6-sulfoquinovose QuiS, consistent with the tribranched hexasaccharide previously reported in the cytochrome of S. acidocaldarius. S. acidocaldarius is the only archaeal species whose N-glycans are known to be linked via the chitobiose core disaccharide that characterises the N-linked glycans of Eukarya.





Autor: Elham Peyfoon, Benjamin Meyer, Paul G. Hitchen, Maria Panico, Howard R. Morris, Stuart M. Haslam, Sonja-Verena Albers, and

Fuente: https://www.hindawi.com/



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