Production and Optimization of Cellulase Enzyme Using Aspergillus niger USM AI 1 and Comparison with Trichoderma reesei via Solid State Fermentation SystemReportar como inadecuado




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Biotechnology Research InternationalVolume 2011 2011, Article ID 658493, 6 pages

Research Article

Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Minden,11800 Penang, Malaysia

Faculty of Agroindustry and Natural Resource, Universiti Malaysia Kelantan, Karung Berkunci 36, Pengkalan Chepa, 16100 Kelantan, Malaysia

Received 8 June 2010; Revised 7 August 2010; Accepted 22 September 2010

Academic Editor: Manuel Canovas

Copyright © 2011 C. K. Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Novel design solid state bioreactor, FERMSOSTAT, had been evaluated in cellulase production studies using local isolate Aspergillus niger USM AI 1 grown on sugarcane bagasse and palm kernel cake at 1 : 1 w-w ratio. Under optimised SSF conditions of 0.5 kg substrate; 70% w-w moisture content; ; aeration at 4 L- fermented substrate for 5 min and mixing at 0.5 rpm for 5 min, about 3.4 U-g of Filter paper activity FPase was obtained. At the same time, comparative studies of the enzymes production under the same SSF conditions indicated that FPase produced by A. niger USM AI 1 was about 35.3% higher compared to Trichoderma reesei. This shows that the performance of this newly designed SSF bioreactor is acceptable and potentially used as prototype for larger-scale bioreactor design.





Autor: C. K. Lee, I. Darah, and C. O. Ibrahim

Fuente: https://www.hindawi.com/



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