Age-Related Alterations in Endothelial Function of Femoral Artery in Young SHR and WKY RatsReport as inadecuate

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BioMed Research InternationalVolume 2014 2014, Article ID 658479, 12 pages

Research Article

Institute of Normal and Pathological Physiology, Centre of Excellence for Examination of Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia

Institute of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University, Mala Hora 4, 036 01 Martin, Slovakia

Received 12 December 2013; Accepted 10 February 2014; Published 20 March 2014

Academic Editor: Ramaroson Andriantsitohaina

Copyright © 2014 Angelika Puzserova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present study was designed to evaluate the effects of vascular aging in juvenescence on endothelial function in femoral arteries and to assess differences between normotensive and hypertensive rats. The aim of the study was to determine if age affected nitric oxide- NO- mediated relaxations in normotensive and hypertensive rats. Juvenile 7-week-old and young adult 22-week-old male Wistar-Kyoto WKY and spontaneously hypertensive rats SHR were used in this study. Femoral artery FA reactivity was determined by wire myograph and NO synthase activity by conversion of

H-L-arginine. During juvenescence systolic blood pressure tail-cuff increased significantly only in SHR, while NO synthesis decreased significantly in both strains. Endothelium-dependent relaxations to acetylcholine were reduced in the FA of SHR compared to age-matched WKY at both ages, yet these parameters were unchanged in adult rats compared with juvenile animals. The NO-dependent component of vasorelaxation was markedly reduced, whereas the NO-independent component was increased in adult compared to juvenile rats in both strains. The endothelial dysfunction in SHR at both ages was associated with reduction of NO-independent mechanisms. In conclusion, aging in early periods of life was associated with reduction of vascular NO production and bioavailability in both strains investigated. This reduction was however fully compensated by accentuation of NO-independent mechanisms.

Author: Angelika Puzserova, Veronika Ilovska, Peter Balis, Peter Slezak, and Iveta Bernatova



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