Depletion of Arginine by Recombinant Arginine Deiminase Induces nNOS-Activated Neurotoxicity in Neuroblastoma CellsReportar como inadecuado

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BioMed Research International - Volume 2014 2014, Article ID 589424, 8 pages -

Research Article

School of Pharmacy, College of Medicine, National Taiwan University, Taipei 10050, Taiwan

Graduate Institute of Clinical Pharmacy, Medical College, National Taiwan University, Taipei 10050, Taiwan

Department of Pharmacy, National Taiwan University Hospital, Taipei 10048, Taiwan

Received 10 February 2014; Revised 25 June 2014; Accepted 27 June 2014; Published 14 July 2014

Academic Editor: Roberta Chiaraluce

Copyright © 2014 Shan-Erh Lin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The abnormal regulation of inducible nitric oxide synthase iNOS and neuronal nitric oxide synthase nNOS is associated with neurodegenerative disorders. Recombinant arginine deiminase rADI is a selective NO modulator of iNOS and eNOS in endothelial cells, and it also exhibits neuroprotective activity in an iNOS-induced neuron-microglia coculture system. However, the effect of rADI on nNOS remains unknown. Addressing this issue is important for evaluating the potential application of rADI in neurodegenerative diseases. SH-SY5Y cells were treated with N-methyl-D-aspartic acid NMDA to activate nNOS. NMDA increased NO production by 39.7 ± 3.9% via nNOS under arginine-containing conditions, but there was no significant increase in both arginine-free and rADI pretreated arginine-containing citrulline buffer. Subsequently, neither NMDA nor rADI alone caused cytotoxicity, whereas cotreatment with NMDA and rADI resulted in dissipation of the cell mitochondrial membrane potential and decreased cell viability. The mechanism of rADI cytotoxicity in the presence of NMDA is caused by the inhibition of NO production via nNOS mediated by the NMDA receptor, which was abolished when extracellular arginine was absent, even in the presence of citrulline. rADI not only reduced NO production but also caused cellular toxicity in nNOS-activated SH-SY5Y cells, suggesting a dual role for rADI in NOS-mediated neurotoxicity.

Autor: Shan-Erh Lin, Fe-Lin Lin Wu, Ming-Feng Wei, and Li-Jiuan Shen



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