Electrospun Gelatin-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2 -Sensing Receptor SignalingReportar como inadecuado




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Stem Cells International - Volume 2015 2015, Article ID 507154, 13 pages -

Research Article

Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China

Department of Dental Materials, Peking University School and Hospital of Stomatology, Beijing 100081, China

Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China

National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing 100081, China

Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing 100081, China

Received 9 January 2015; Revised 17 February 2015; Accepted 17 February 2015

Academic Editor: Kee Woei Ng

Copyright © 2015 Xuehui Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Calcium phosphate- CaP- based composite scaffolds have been used extensively for the bone regeneration in bone tissue engineering. Previously, we developed a biomimetic composite nanofibrous membrane of gelatin-β-tricalcium phosphate TCP and confirmed their biological activity in vitro and bone regeneration in vivo. However, how these composite nanofibers promote the osteogenic differentiation of bone marrow mesenchymal stem cells BMSCs is unknown. Here, gelatin-β-TCP composite nanofibers were fabricated by incorporating 20 wt% β-TCP nanoparticles into electrospun gelatin nanofibers. Electron microscopy showed that the composite β-TCP nanofibers had a nonwoven structure with a porous network and a rough surface. Spectral analyses confirmed the presence and chemical stability of the β-TCP and gelatin components. Compared with pure gelatin nanofibers, gelatin-β-TCP composite nanofibers caused increased cell attachment, proliferation, alkaline phosphatase activity, and osteogenic gene expression in rat BMSCs. Interestingly, the expression level of the calcium-sensing receptor CaSR was significantly higher on the composite nanofibrous scaffolds than on pure gelatin. For rat calvarial critical sized defects, more extensive osteogenesis and neovascularization occurred in the composite scaffolds group compared with the gelatin group. Thus, gelatin-β-TCP composite scaffolds promote osteogenic differentiation of BMSCs in vitro and bone regeneration in vivo by activating Ca

-sensing receptor signaling.





Autor: Xuehui Zhang, Song Meng, Ying Huang, Mingming Xu, Ying He, Hong Lin, Jianmin Han, Yuan Chai, Yan Wei, and Xuliang Deng

Fuente: https://www.hindawi.com/



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